Wuchereria bancrofti recombinant antigen-derived poly- and monoclonal antibodies for the detection of circulating antigen(s) in the sera of lymphatic filarial patients.
Identifieur interne : 00BC60 ( Main/Exploration ); précédent : 00BC59; suivant : 00BC61Wuchereria bancrofti recombinant antigen-derived poly- and monoclonal antibodies for the detection of circulating antigen(s) in the sera of lymphatic filarial patients.
Auteurs : J G Theodore [Inde] ; P. KalirajSource :
- Journal of helminthology [ 0022-149X ] ; 1996.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antihelminthe (immunologie), Anticorps monoclonaux (immunologie), Antigènes d'helminthe (immunologie), Antigènes d'helminthe (sang), Bovins, Filariose lymphatique (diagnostic), Filariose lymphatique (immunologie), Filariose lymphatique (sang), Humains, Lapins, Microfilaria (immunologie), Protéines recombinantes (immunologie), Réactions croisées, Setaria (nématode) (immunologie), Souris, Test ELISA, Wuchereria bancrofti (immunologie).
- MESH :
- diagnostic : Filariose lymphatique.
- immunologie : Anticorps antihelminthe, Anticorps monoclonaux, Antigènes d'helminthe, Filariose lymphatique, Microfilaria, Protéines recombinantes, Setaria (nématode), Wuchereria bancrofti.
- sang : Antigènes d'helminthe, Filariose lymphatique.
- Animaux, Bovins, Humains, Lapins, Réactions croisées, Souris, Test ELISA.
English descriptors
- KwdEn :
- Animals, Antibodies, Helminth (immunology), Antibodies, Monoclonal (immunology), Antigens, Helminth (blood), Antigens, Helminth (immunology), Cattle, Cross Reactions, Elephantiasis, Filarial (blood), Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (immunology), Enzyme-Linked Immunosorbent Assay, Humans, Mice, Microfilariae (immunology), Rabbits, Recombinant Proteins (immunology), Setaria Nematode (immunology), Wuchereria bancrofti (immunology).
- MESH :
- chemical , blood : Antigens, Helminth.
- chemical , immunology : Antibodies, Helminth, Antibodies, Monoclonal, Antigens, Helminth, Recombinant Proteins.
- blood : Elephantiasis, Filarial.
- diagnosis : Elephantiasis, Filarial.
- immunology : Elephantiasis, Filarial, Microfilariae, Setaria Nematode, Wuchereria bancrofti.
- Animals, Cattle, Cross Reactions, Enzyme-Linked Immunosorbent Assay, Humans, Mice, Rabbits.
Abstract
A sandwich antibody ELISA was employed for the detection of circulating filarial antigen in patients with bancroftian filariasis. Wuchereria bancrofti recombinant antigen-derived polyclonal and monoclonal antibodies were successfully used as the revealing antibodies and their efficiency was compared. All the microfilariae (mf) positive (by finger prick and examination of 20 microliters of blood under the microscope) individuals tested showed the presence of circulating antigen(s). Among the antigen positive endemic normals (mf negative by the finger prick method), 43% showed microfilariae by a sensitive parasitological method viz. membrane filtration of the night blood samples. A significant correlation was observed between the parasite antigen levels and the blood microfilaria counts among the mf carriers. This information on the parasite antigen levels could be an ideal monitor to indicate the degree of active infection and in the follow up of chemotherapy.
PubMed: 8960201
Affiliations:
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Le document en format XML
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Kaliraj</name></author></analytic><series><title level="j">Journal of helminthology</title><idno type="ISSN">0022-149X</idno><imprint><date when="1996" type="published">1996</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Helminth (immunology)</term><term>Antibodies, Monoclonal (immunology)</term><term>Antigens, Helminth (blood)</term><term>Antigens, Helminth (immunology)</term><term>Cattle</term><term>Cross Reactions</term><term>Elephantiasis, Filarial (blood)</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Elephantiasis, Filarial (immunology)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term><term>Mice</term><term>Microfilariae (immunology)</term><term>Rabbits</term><term>Recombinant Proteins (immunology)</term><term>Setaria Nematode (immunology)</term><term>Wuchereria bancrofti (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antihelminthe (immunologie)</term><term>Anticorps monoclonaux (immunologie)</term><term>Antigènes d'helminthe (immunologie)</term><term>Antigènes d'helminthe (sang)</term><term>Bovins</term><term>Filariose lymphatique (diagnostic)</term><term>Filariose lymphatique (immunologie)</term><term>Filariose lymphatique (sang)</term><term>Humains</term><term>Lapins</term><term>Microfilaria (immunologie)</term><term>Protéines recombinantes (immunologie)</term><term>Réactions croisées</term><term>Setaria (nématode) (immunologie)</term><term>Souris</term><term>Test ELISA</term><term>Wuchereria bancrofti (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antigens, Helminth</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Helminth</term><term>Antibodies, Monoclonal</term><term>Antigens, Helminth</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antihelminthe</term><term>Anticorps monoclonaux</term><term>Antigènes d'helminthe</term><term>Filariose lymphatique</term><term>Microfilaria</term><term>Protéines recombinantes</term><term>Setaria (nématode)</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Elephantiasis, Filarial</term><term>Microfilariae</term><term>Setaria Nematode</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Antigènes d'helminthe</term><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cattle</term><term>Cross Reactions</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term><term>Mice</term><term>Rabbits</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Bovins</term><term>Humains</term><term>Lapins</term><term>Réactions croisées</term><term>Souris</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A sandwich antibody ELISA was employed for the detection of circulating filarial antigen in patients with bancroftian filariasis. Wuchereria bancrofti recombinant antigen-derived polyclonal and monoclonal antibodies were successfully used as the revealing antibodies and their efficiency was compared. All the microfilariae (mf) positive (by finger prick and examination of 20 microliters of blood under the microscope) individuals tested showed the presence of circulating antigen(s). Among the antigen positive endemic normals (mf negative by the finger prick method), 43% showed microfilariae by a sensitive parasitological method viz. membrane filtration of the night blood samples. A significant correlation was observed between the parasite antigen levels and the blood microfilaria counts among the mf carriers. This information on the parasite antigen levels could be an ideal monitor to indicate the degree of active infection and in the follow up of chemotherapy.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Kaliraj, P" sort="Kaliraj, P" uniqKey="Kaliraj P" first="P" last="Kaliraj">P. Kaliraj</name></noCountry><country name="Inde"><noRegion><name sortKey="Theodore, J G" sort="Theodore, J G" uniqKey="Theodore J" first="J G" last="Theodore">J G Theodore</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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